Fluorescent dyes can be used to detect various biological substances in tissues and are indispensable for understanding biological phenomena. However, some substances in living organisms emit fluorescence as noise, which is difficult to distinguish from artificially labeled fluorescence. While recent advances in detection technology have led to higher sensitivity and resolution in fluorescence detection, little progress has been made in dealing with autofluorescence as noise, and new quenching technology has been long awaited. The research group has been developing “quenching technology by light irradiation,” a completely different approach from the reagent-based quenching technology that has been mainly used until now. This new technology has succeeded in quenching autofluorescence practically in a short period of time. By using this device prior to fluorescence staining, it is possible to eliminate only tissue-derived autofluorescence noise without degrading the labeled fluorescence signal. The device can also be used after fluorescence staining to return the sample to its pre-stained state, making it possible to perform fluorescence staining of the same tissue sample multiple times.
Frontiers in Molecular Neuroscience, September 2, 2022 issue
Fluorescence quenching by high-power LEDs for highly sensitive fluorescence in situ hybridization.
Yousuke Tsuneoka*, Yusuke Atsumi, Aki Makanae, Mitsuru Yashiro, Hiromasa Funato*